YES. You can convert your python project that uses tkinter into a standarlone executable app which includes dependencies inside. py2app is one of the popular methods to make python apps.

This article might help you　--->　𒅌𒋨 (Remember to include your packages inside the package list before running the build process!)

After that, you can upload the .app or .exe file to your website for the public. If you wanna publish it in Apple's AppStore, there is some more regulations. Eg. your age has to be 18+.

As clarification to @Abdelmasieh Baselious's answer, MacOS allows apps from unknown sources.