Based on the stack trace provided, on _layouts.cshtml, the file is referencing an optimization bundle that includes the path ".../App/Controllers", i am assuming you have verified that the directory exists. i am thinking, the line 21 mentioned in the trace, is likely a Scripts.Render() or Styles.Render() which i think would be rendering from "~bundles/app", can you check to see in your BundleConfig.cs what's that mapped too and if any *.js are in that "../Controllers" directory?