I have just quick tested your settings. Created new canvas with one image filling the screen second image with rect tr. top and bottom on 450 and copying rest of what you have and the result is that gamemode UI perfectly matches the scene view one. Only difference I see that your canvas Rect Transform have scale 0,51 The scale could be problem I remember first time fiddling out with UI and changing canvas size but here if I change it manually to 0,51 I still have correct result. But I also see that you have height to 974,31 and if I change that, then the stripe will become uneven. I just dont understand how it got messed up when you have correctly Screen Space Overlay